Guide Signalling by inositides : a practical approach

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  1. A Mathematical Model of the Phosphoinositide Pathway | Scientific Reports
  2. A Mathematical Model of the Phosphoinositide Pathway
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  4. The physiology and pathology of inositide signaling in the nucleus
  5. Signalling by Inositides: A Practical Approach (Practical Approach Series)

Click on an option below to access. Log out of ReadCube. The understanding of its biological role is supported by the recent acquisitions dealing with pathology and namely hematological malignancies. Here, we review this issue highlighting the main achievements in the last years.

A Mathematical Model of the Phosphoinositide Pathway | Scientific Reports

Volume , Issue 1. The full text of this article hosted at iucr. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account. If the address matches an existing account you will receive an email with instructions to retrieve your username. Lucio Cocco Corresponding Author E-mail address: lucio.

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A Mathematical Model of the Phosphoinositide Pathway

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Purchase Instant Access. View Preview. Learn more Check out. The best-characterized inositol pyrophosphates are the diphosphoinositol pentakisphosphate IP7 and the bis-diphosphoinositol tetrakisphosphate IP8. Our ability to produce radiolabeled IP7 with high specific activity has allowed us to detect the direct transfer of the radiolabel beta phosphate from IP7 to proteins. This phosphorylation mechanism defines a yet new kind of protein post-translational modification.


A recent study has shown that inositol pyrophosphates donate the phosphate group to pre-phosphorylated serines, thus generating a pyro-phosphorylated protein. Although we are able to reproducibly transfer phosphate moieties from IP7 to pre-phosphorylated serine of proteins in vitro and have indirect evidence that IP7 can directly mediate phosphorylation in vivo, the molecular mechanisms underlying IP7-dependent protein pyro-phosphorylation in vivo and the physiological consequence of such, are not fully elucidated yet.

The main objective of my laboratory is to identify the physiological functions of inositol pyro-phosphates.

The physiology and pathology of inositide signaling in the nucleus

We are employing several experimental models from yeast Saccharomyces cerevisiae, a simple but powerful genetic model, to mouse knockout to mimic human physiology. We are routinely using several biochemical, molecular biology and cell biology techniques. It has become increasingly clear that inositol pyrophosphates have a very basic signalling function inside all eukaryotic cells. It is likely that the inositol pyro-phosphates have evolved dedicated roles due to their unique biochemistry.

The potential therapeutic potential of such research is supported by the recent identification of the critical role inositol pyrophosphates in the regulation of insulin secretion and oncogenic processes. We have substantially contributed to both studies. Publications The following are buttons which change the sort order, pressing the active button will toggle the sort order Author Name descending press to sort ascending.

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Azevedo C The new world of inorganic polyphosphates. Azevedo C Functions of inorganic polyphosphates in eukaryotic cells: a coat of many colours. Azevedo C Extraction and analysis of soluble inositol polyphosphates from yeast. Azevedo C Protein polyphosphorylation of lysine residues by inorganic polyphosphate. This poster will be sent to institutions around Europe for display.

Inositol Triphosphate (IP3) and Calcium Signaling Pathway - Second Messenger System

Can be use to identify IP6K inhibitors. Although this technology was just published in May by October the majority of scientists working on inositol pyrophosphate research have adopted this new method. I hosted several students and postdoc from around the world in my lab to directly learn this powerful technology. Open Biol. Many paper are coming out using this methods that have helped to develop new chemical tools, such: Prometabolites of 5-Diphospho-myo-inositol Pentakisphosphate. Angew Chem Int Ed Engl. Collaborator Contribution They have allowed me to study signaling by inositol phosphates in neuronal cell.

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Neurons are an excellent experimental system to study signaling mechanism that due to technical difficulty is to often misuse or not use. Thanks to this collaboration I now using neuronal cell as experimental model. Collaborator Contribution This partnership opens the unique possibility to direct analyze-imagining inositol inside cells. Claude with his expertise and technology will allow unique investigation.

Impact This collaboration has allowed me to win a very prestigious HFSP grant thus enhancing economically and scientifically the profile of the lab.

Signalling by Inositides: A Practical Approach (Practical Approach Series)

At the moment the only outputs this collaboration generated is extra money and higher scientific profile for the lab. Prometabolites of 5-Diphospho-myo-inositol Pentakisphosphate. PMID: Our experience in inositol polyphosphate and polyP analysis have rapidly facilitated the development of this model thanks to the help of our collaborator.

We are now using amoeba to understand the role of the cited molecules to control cellular phosphate homeostasis. Collaborator Contribution He transferred his know-how on Amoeba biology to my laboratory personnel, furthermore, he has given us tools and reagents essential to initiate to perform Dictyostelium discoideum research.

Developmental accumulation of inorganic polyphosphate affects germination and energetic metabolism in Dictyostelium discoideum. Analysis of Dictyostelium discoideum inositol pyrophosphate metabolism by gel electrophoresis. PLoS One. Biochemical analysis of Schizosaccharomyces pombe inositol kinases mutant strains. Collaborator Contribution Initiated the project identifying by genetic studies key roles for inositol phosphate kinases of Schizosaccharomyces pombe, Is providing stains, expertise, tools and genetic analyses. Impact This collaboration is just started, thus there are not output to be listed.

However, a important publication has been just been accepted Mol Cell Biol.